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Papers of the Week


Papers: 29 Oct 2022 - 4 Nov 2022


2022 Oct 05


Bio Protoc


12


19

LIST: A Newly Developed Laser-assisted Cell Bioprinting Technology.

Authors

Roversi K, Orimi H E, Erfanian M, Talbot S, Boutopoulos C
Bio Protoc. 2022 Oct 05; 12(19).
PMID: 36313198.

Abstract

Cell bioprinting technologies aim to fabricate tissue-like constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. We recently introduced a laser-assisted drop-on-demand bioprinting technology termed Laser Induced Side Transfer (LIST). This technology can print delicate cell types, including primary neurons. This bioprinting protocol includes the following key steps: cell harvesting, bio-ink preparation, laser setup priming, printing, and post-printing analysis. This protocol includes a detailed description of the laser setup, which is a rather unusual setup for a biology lab. This should allow easy reproduction by readers with basic knowledge of optics. Although we have focused on neuron bioprinting, interested readers will be able to adapt the protocol to bioprint virtually any cell type.