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Papers of the Week


Papers: 23 Apr 2022 - 29 Apr 2022


2022


Front Cell Neurosci


16

The T-Type Calcium Channel Cav3.2 in Somatostatin Interneurons in Spinal Dorsal Horn Participates in Mechanosensation and Mechanical Allodynia in Mice.

Authors

Zhi Y-R, Cao F, Su X-J, Gao S-W, Zheng H-N, Jiang J-Y, Su L, Liu J, Wang Y, Zhang Y, Zhang Y
Front Cell Neurosci. 2022; 16:875726.
PMID: 35465611.

Abstract

Somatostatin-positive (SOM) neurons have been proposed as one of the key populations of excitatory interneurons in the spinal dorsal horn involved in mechanical pain. However, the molecular mechanism for their role in pain modulation remains unknown. Here, we showed that the T-type calcium channel Cav3.2 was highly expressed in spinal SOM interneurons. Colocalization of (which codes for Cav3.2) and SOM was observed in the hybridization studies. Fluorescence-activated cell sorting of SOM cells in spinal dorsal horn also proved a high expression of in SOM neurons. Behaviorally, virus-mediated knockdown of in spinal SOM neurons reduced the sensitivity to light touch and responsiveness to noxious mechanical stimuli in naïve mice. Furthermore, knockdown of in spinal SOM neurons attenuated thermal hyperalgesia and dynamic allodynia in the complete Freund's adjuvant-induced inflammatory pain model, and reduced both dynamic and static allodynia in a neuropathic pain model of spared nerve injury. Mechanistically, a decrease in the percentage of neurons with Aβ-eEPSCs and Aβ-eAPs in superficial dorsal horn was observed after knockdown in spinal SOM neurons. Altogether, our results proved a crucial role of Cav3.2 in spinal SOM neurons in mechanosensation under basal conditions and in mechanical allodynia under pathological pain conditions. This work reveals a molecular basis for SOM neurons in transmitting mechanical pain and shows a functional role of Cav3.2 in tactile and pain processing at the level of spinal cord in addition to its well-established peripheral role.